P9599
Sigma-Aldrich

Protease Inhibitor Cocktail

Nacres: NA.77
Mdl No: MFCD00677817
Grouped product items
SKU Pack Size Availability Price Quantity
Request a Bulk Order Check Availability

Properties

Properties
Description Protease Inhibitor Cocktail for plant cell and tissue extracts, DMSO solution
form DMSO solution
MDM Code P9599-5ML
Material Number P9599
Business Unit 687
CAS Number 0

Description

Application

Optimized and tested for use with plant tissue and cell extracts. Specific testing was done on plant seedling extracts from pea, bean, wheat, tobacco, and Arabidopsis, as well as leaf and root extracts from pea, wheat and tobacco.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF, 1,10-Phenanthroline, Pepstatin A, Leupeptin, Bestatin, and E-64. Each component has specific inhibitory properties. AEBSF acts to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases. 1,10-Phenanthroline acts against metalloproteases.

Caution

The cocktail should be stored at -20°C, where it will retain stability for two years.

Preparation Note

This product is supplied as a clear, faint pink solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells. Extracts of plant seedlings from pea, bean, wheat, tobacco, and Arabidopsis have been tested. The roots of these plants have also been successfully tested.

Quantity

One mL is recommended for the inhibition of proteases extracted from 30 g of plant tissue in a total volume of 100 ml.

Specificity

Inhibits serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 1

Flash Point (F)

185.0 °F - closed cup

Flash Point (C)

85 °C - closed cup

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Welcome to Merck Ecommerce (Beta). During this Beta phase, you may encounter occasional broken function, layout and issues. ×