P6486
Millipore

EZview(TM) Red Protein A Affinity Gel

Nacres: Red Protein A Affinity Gel
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Properties

Properties
analyte chemical classes proteins (Immunoglobulins of various mammalian species)
capacity ~4 
capacity × capacity mg binding capacity (rabbit IgG)
Description EZview(TM) Red Protein A Affinity Gel
format liquid
MDM Code P6486-1ML
Material Number P6486
Business Unit 687
CAS Number 0

Description

Application

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

General description

EZview Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 

Physical properties

ppm Kathon

Subculture Routine

EZview

Substrates

Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 1

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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