L6876
Sigma-Aldrich

Lysozyme from chicken egg white

Synonyms English:

Mucopeptide N-acetylmuramoylhydrolase

Mdl No: MFCD00131557
CAS Number: 12650-88-3
Grouped product items
SKU Pack Size Availability Price Quantity
Request a Bulk Order Check Availability

Properties

Properties
composition protein ≥
compression i.d. 90%
Description Lysozyme from chicken egg white lyophilized powder, protein >=90 %, >=40,000 units/mg protein
format lyophilized powder
glass tube l × o.d. × i.d. chicken ... LYZ(396218)
MDM Code L6876-25G
Material Number L6876
Business Unit 687
Shipping Mode AP
MRP Type PD
Local HS Code 35079000
EUD NO
Legacy SIGMA
Shelf Life 0
Plant 6311
Sales Org 6304
EUD Documents Needed 0
CAS Number 12650-88-3

Description

Application

Enzyme breaks down the cell walls of bacteria

Biochem/physiol Actions

used to prepare spheroplasts.

Caution

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Warning

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

SAFETY INFORMATION

Signal Word

Danger

P261 - P284 - P501

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Welcome to Merck Ecommerce (Beta). During this Beta phase, you may encounter occasional broken function, layout and issues. ×