D9779
Sigma-Aldrich

DL-Dithiothreitol

Linear Formula:

HSCH2CH(OH)CH(OH)CH2SH

Empform:

C4H10O2S2

Synonyms English:

threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT

Nacres: NA.31
Mdl No: MFCD00004877
Fw: 154.25
Pubchem Substance Id: 24894330
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Properties

Properties
assay
assay range 98% (HPLC)
cavity d heavy metals (as Pb): ≤
cavity diam.
cavity diam. × d ppm
Description DL-Dithiothreitol for molecular biology, >=98% (HPLC), >=99% (titration)
fragrance allergen DNase RNase protease none detected
frame l × w powder
grain size for molecular biology
inherent viscosity 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H1-2H2/t3-4-/m1/s1
inlet and outlet diam. VHJLVAABSRFDPM-QWWZWVQMSA-N
MDM Code D9779-250MG
Material Number D9779
Business Unit 499
Shipping Mode AP
MRP Type PD
Local HS Code 29309090
EUD NO
Legacy SIGMA
Shelf Life 0
Plant 6311
Sales Org 6304
EUD Documents Needed 0

Description

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

General description

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

SAFETY INFORMATION

GHS05,GHS07

Signal Word

Danger

H302 - H315 - H318

P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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